一：概述

  點(diǎn)擊化學(xué)(Click chemistry)，又譯為"鏈接化學(xué)"、"速配接合組合式化學(xué).二苯并環(huán)辛炔（ADIBO，DBCO）是用于應(yīng)變促進(jìn)的炔疊氮化物環(huán)加成（spAAC）（一種無(wú)銅Click化學(xué)反應(yīng)）的*具反應(yīng)性的環(huán)炔。

這是一種胺反應(yīng)性NHS酯，可將反應(yīng)性部分輕松連接*幾乎任何伯或仲胺基團(tuán)，例如蛋白質(zhì)，肽或小分子胺基團(tuán)。

DBCO立即與疊氮化物反應(yīng)。該反應(yīng)速率遠(yuǎn)高于銅催化的反應(yīng)速率以及與許多其他環(huán)辛炔的反應(yīng)速率。與某些其他環(huán)辛炔不同，DBCO不與四嗪發(fā)生反應(yīng)-這允許疊氮化物與DBCO和反式環(huán)辛烯與四嗪進(jìn)行正交共軛。

二：原理

點(diǎn)擊化學(xué)反應(yīng)主要有4 種類(lèi)型

1.環(huán)加成反應(yīng)

2.親核開(kāi)環(huán)反應(yīng)

3.非醇醛的羰基化學(xué)

4.碳碳多鍵的加成反應(yīng)

三：反應(yīng)特征

1. 反應(yīng)原料容易得到
2. 對(duì)于氧氣、水不敏感
3. 產(chǎn)率相對(duì)較高
4. 產(chǎn)物的立體選擇性好

DBCO胺反應(yīng)試劑和DBCO-PEG試劑：

DBCO-C6-NHS Ester           

DBCO-NHS Ester                 

DBCO-Sulfo-NHS Ester

DBCO-PEG4-NHS Ester            

DBCO-STP Ester

DBCO-PEG5-NHS Ester            

DBCO-PEG5-TFP Ester

DBCO Acid                    

DBCO-C6-Acid                  

DBCO-PEG5-Acid    

PC DBCO-NHS Ester  

DBCO Amine                   

DBCO-PEG4-Amine              

Sulfo DBCO-Amine  

Sulfo DBCO-PEG4-Amine

Bis-sulfone-PEG4-DBCO

DBCO Maleimide               

DBCO-PEG4-Maleimide    

Sulfo DBCO-Maleimide

Sulfo DBCO-PEG4-Maleimide

DBCO-PEG-MAL

DBCO-PEG-COOH

DBCO-PEG-NH2

DBCO-PEG-Biotin

DBCO-PEG-Silane

DBCO-PEG-SH

DBCO生物素反應(yīng)試劑:

Biotin-PEG4-DBCO    

WS Biotin DBCO   

Dde Biotin DBCO

Disulfide Biotin DBCO  

Desthiobiotin-PEG4-DBCO   

Diazo Biotin DBCO  

PC DBCO Biotin  
 

DBCO標(biāo)記熒光探針

BCO-Cyanine3

DBCO-Cyanine3.5

DBCO-Disulfo-Cyanine3

DBCO-trisulfo-Cyanine3.5

DBCO-Cyanine5

DBCO-Cyanine5.5

DBCO-Disulfo-Cyanine5

DBCO-trisulfo-Cyanine5.5

DBCO-Cyanine7

DBCO-ICG

DBCO-trisulfo-ICG

AFDye 405 DBCO   

AFDye 430 DBCO     

AFDye 488 DBCO     

AFDye 532 DBCO     

AFDye 546 DBCO    

AFDye 555 DBCO     

AFDye 568 DBCO      

AFDye 594 DBCO     

AFDye 647 DBCO    

Cy3 DBCO           

Cy5 DBCO          

Cy5.5 DBCO        

Cy7 DBCO           

MB 488 DBCO       

MB 543 DBCO     

MB 594 DBCO     

MB 660R DBCO        

MB 680R DBCO        

TAMRA DBCO        

小分子DBCO產(chǎn)品：

DBCO-PEG4-NHS Ester

DBCO-PEG5-NHS Ester

DBCO-PEG5-TFP Ester

DBCO-PEG4-Amine

Sulfo DBCO-PEG4-Amine

Bis-sulfone-PEG4-DBCO

Biotin-PEG4-DBCO

Desthiobiotin-PEG4-DBCO

DBCO-PEG4-DBCO

DBCO-PEG4-OH

DBCO-C4-COOH

Sulfo DBCO-PEG4-Maleimide

DBCO-PEG4-Maleimide

DBCO-PEG5-Acid    

DBCO-C6-Acid