Kerafast ENH001 Anti-DNA-RNA Hybrid [S9.6] Antibody

時(shí)間：2022/10/1閱讀：175

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靶點(diǎn)科技（北京）有限公司
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Kerafast ENH001 Anti-DNA-RNA Hybrid [S9.6] Antibody 針對(duì)ΦX174噬菌體衍生的合成DNA-RNA抗原產(chǎn)生該小鼠單克隆抗體，并識(shí)別各種長(zhǎng)度的RNA-DNA雜交體。

產(chǎn)品特色：

對(duì)DNA-RNA雜交體具有高度特異性和親和力
可用于檢測(cè)R環(huán)
不與單鏈DNA或雙鏈DNA交叉反應(yīng)
對(duì)于富含AU的雙鏈RNA，已觀察到輕微的交叉反應(yīng)（約5倍）。
對(duì)于長(zhǎng)度為8,10,15和23個(gè)堿基對(duì)的雜交體顯示出高親和力結(jié)合

DNA-RNA雜合體是真核細(xì)胞中的天然存在，這些za種的水平在具有高轉(zhuǎn)錄活性的位點(diǎn)增加，例如在轉(zhuǎn)錄起始，抑制和延伸期間。由于RNA-DNA雜交體影響基因組不穩(wěn)定性，S9.6抗體是一種有用的試劑，可幫助研究這些za種在DNA復(fù)制或其他細(xì)胞過程中形成的R環(huán)和損傷的后果。此外，S9.6抗體可有效識(shí)別用于微陣列研究的RNA-DNA雜交。

背景介紹：

DNA-RNA hybrids are a natural occurrence within eukaryotic cells, with levels of these hybrids increasing at sites with high transcriptional activity, such as during transcription initiation, repression, and elongation. Because RNA-DNA hybrids influence genomic instability, the S9.6 antibody is a useful reagent to help study the consequences of R-loops and lesions formed by these hybrids during DNA replication or other cellular processes. In addition, the S9.6 antibody is effective in recognizing RNA-DNA hybridization for microarray studies。

產(chǎn)品詳情：

Product Type:	Antibody
Name:	Anti-DNA-RNA Hybrid [S9.6]
Antigen:	S9.6 ΦX174 bacteriophage-derived synthetic DNA–RNA antigen
Isotype:	Rabbit IgG
Fusion Tag(s):	Mouse Fab version contains His-tag
Clone Name:	S9.6
Reactivity:	High specificity and affinity for DNA/RNA hybrids and other A-form nucleic acid hybrids
Immunogen:	ΦX174 bacteriophage-derived synthetic DNA/RNA
Purification Method:	Protein A/G
Buffer:	ENHOO1: PBS, 0.05% (w/v) Sodium Azide Ab01137- : PBS with 0.02% Proclin 300
Tested Applications:	Dot Blot Analysis: 0.2 µg/mL. Affinity Binding Assay: Clone S9.6 bound the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. Single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA were not bound by clone S9.6 (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected increased DNA RNA hybrids at four actively transcribed genes upon shRNA-mediated knockdown of BRCA1 or BRCA2, but not PCID2 or RAD51 in HeLa cells (Bhatia, V., et al. (2014). Nature. 511(7509):362-365). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected R-loops formed over beta-actin gene using HeLa chromatin preparation. RNase H treatment of the chromatin preparation prevented clone S9.6 from immunoprecipitating target chromatin fragments (Skourti-Stathaki, K., et al. (2011). Mol. Cell. 42(6):794-805). Chromatin Immunoprecipitation-sequencing (ChIP-seq) Analysis: A representative lot detected genome-wide distribution of DNA-RNA hybrids in budding yeast by ChIP-seq analysis (El Hage, A., et al. (2014). PLoS Genet. 10(10):e1004716). Immunocytochemistry Analysis: Representative lots immunolocalized nuclear R loops by fluorescent immunocytochemistry staining of methanol-fixed H1 human embryonic stem cells (hESCs) and formaldehyde-fixed HeLa cells (Bhatia, V., et al. (2014). Nature. 511(7509):362-365; Ginno, P.A., et al. (2012). Mol. Cell. 45(6):814-825). Immunoprecipitation Analysis: A representative lot immunoprecipitated in vitro transcribed R-loop substrate (DNA-RNA hybrid), but not doouble-stranded DNA (dsDNA) (Ginno, P.A., et al. (2012). Mol. Cell. 45(6):814-825).

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靶點(diǎn)科技（北京）有限公司

靶點(diǎn)科技（北京）有限公司

Kerafast ENH001 Anti-DNA-RNA Hybrid [S9.6] Antibody

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