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RPMI-1640培養(yǎng)基更適于腫瘤細胞體外培養(yǎng)

時間:2013-1-23閱讀:4029
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 進行腫瘤細胞體外培養(yǎng)建議RPMI-1640 培養(yǎng)基

RPMI-1640培養(yǎng)基更適于腫瘤細胞體外培養(yǎng)

      DMEM 和RPMI-1640 是兩種zui常用的商品化培養(yǎng)基。培養(yǎng)基中細胞的 生長速度時快時慢,還有學(xué)者發(fā)現(xiàn)培養(yǎng)液不同,細胞的生長速度不盡相同。
      但對于適宜細胞 生長培養(yǎng)基的選擇、細胞數(shù)量以及培養(yǎng)時間對細胞生長增殖狀態(tài)的影響有待進一步研究。
      培養(yǎng)基是細胞賴以體外生長、增殖、分化的重要因素,細胞生長分泌過程需要大量營養(yǎng) 物質(zhì)。氨基酸作為培養(yǎng)基中的重要成分,對于細胞的生長起著明顯的促進作用。

      進行腫瘤細胞體外培養(yǎng)建議RPMI-1640 培養(yǎng)基。

     美國GIBCO公司RPMI-1640和DMEM培養(yǎng)基其成分對比如下。

培養(yǎng)基成分:

              成分           PRMI  1640(mg/L )        DMEM(mg/L )

精氨酸20084
天冬酰胺50-
天冬氨酸20-
胱氨酸-48
半胱氨酸50-
谷氨酸20-
谷氨酰胺300584
甘氨酸1030
組氨酸1542
羥脯氨酸20-
異亮氨酸50104.8
亮氨酸50104.8
賴氨酸40146.2
蛋氨酸1530
苯丙氨酸1566
脯氨酸20-
絲氨酸3042
蘇氨酸2095.2
色氨酸516
酪氨酸2072
纈氨酸2093.6
生物素0.2-
偏多酸鈣0.254
氯化膽堿34
葉酸14
肌醇357.2
菸酰胺14
-氨-苯甲酸1-
吡哆醛-4
吡哆醇1-
核黃素0.20.4
硫胺14
維生素B120.005-
CaCl2-200
Ca(NO3)2·4H2O100-
Fe(NO3)3·9H2O-0.1
KCl400400
MgSO448.8200
NaCl60006 400
NaHCO32 0003 700
NaH2PO4·H2O-141.3
Na2HPO4800.7-
葡萄糖2 0004 500
酚紅515
丙酮酸鹽-110
HEPES5958-


               乾思生物(ATCC細胞 ScienCell細胞 CellResearchCorp 原代細胞)已獲得多家科研單位及老師認可,是細胞株、血清、培養(yǎng)基專業(yè)供應(yīng)商,歡迎你的選購。

乾思生物整理編輯: 進口elisa試劑盒,國產(chǎn)elisa試劑盒,elisa試劑盒,試劑盒,dna提取試劑盒,免疫組化試劑盒,rna提取試劑盒,質(zhì)粒提取試劑盒,dna試劑盒

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