目前蛋白質(zhì)組學(xué)耗材成本高昂。
現(xiàn)有蛋白質(zhì)組學(xué)方法需要很高的技術(shù)門(mén)檻。
需要建立簡(jiǎn)單易用和隨時(shí)可用的方法。
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Empore™ E系列技術(shù)簡(jiǎn)介
E3™技術(shù)特點(diǎn)
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E3™產(chǎn)品類(lèi)型
E3filter測(cè)試結(jié)果
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Fig.1 Qualitative and quantitative assessment of E3technology (E3 filter) for E. coli proteome analysis. (A-C) Comparison of the number of proteins, peptides, and PSMs between the E3filter, FASP, and SP4-GB approaches. Error bars represent three replicates. (D-E) Overlapping analyses of proteins and peptides derived from the three methods. (F) Coefficient of variation of quantified proteins by the three methods. (G) Coefficient of variation of quantified peptides. (H) Pearson correlation between replicate experiments and different methods. (I) Percentages of missed cleavages. (J-K) Volcano plot of showing significantly differential proteins between the three methods. The two curves show FDR 0.05 and 0.01, respectively. (L) Heatmap of the overall quantified proteins by the three methods. FASP, filter aided sample preparation; SP4, Solvent Precipitation SP3; GB, glassbeads.
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E3工作流程
E4工作流程
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參考文獻(xiàn)
Yu YB, et al. Cell Reports Methods 4, 2024, 100796, June 17.
Johnston HE, et al.Anal Chem.2022, 94:10320-10328.
Wi?niewskiJR, et al. Nat Methods. 2009,6:359-62.
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