魚雌激素ELISA試劑盒實(shí)驗(yàn)原理：本試劑盒用于測定魚血清，血漿,組織及相關(guān)液體樣本中激素（E）的含量。應(yīng)用雙抗體夾心法測定標(biāo)本中魚雌激素（E）水平。用純化的魚雌激素（E）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入雌激素（E），再與HRP標(biāo)記的雌激素（E）抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的雌激素（E）呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計(jì)算樣品中魚雌激素（E）濃度。
Fish Estrogen(E)ELISA Kit
Purpose
This kit allows for the determination of E concentrations in Fish serum, blood plasma, tissue and other biological fluids.
Principle of the assay
The kit assay Fish E level in the sample，use Purified Fish E to coat microtiter plate wells, make solid-phase antibody, then add E to wells, Combined E antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of E in the samples is then determined by comparing the O.D. of the samples to the standard curve.
試劑盒供應(yīng)商：上海華壹生物科技有限公司