手機(jī)版
化工儀器網(wǎng)手機(jī)版
化工儀器網(wǎng)小程序
官方微信
公眾號(hào)：chem17
掃碼關(guān)注視頻號(hào)
技術(shù)產(chǎn)品文獻(xiàn):豬ALT,AST,ELISA試劑盒引用文獻(xiàn)
【文獻(xiàn)標(biāo)題】Novel insights into cytochrome P450 enzyme and solute carrier families in cadmium-induced liver injury of pigs
【作者】Huan Wang, Qi Han,Yongjie Chen,et.al
【作者單位】東北農(nóng)業(yè)大學(xué)（Northeast Agricultural University）
【文獻(xiàn)中引用產(chǎn)品】
豬丙氨酸轉(zhuǎn)氨酶(ALT)ELISA試劑盒
豬天門冬氨酸氨基轉(zhuǎn)移酶（AST)ELISA試劑盒
【關(guān)鍵詞】Cadmium ,Cytochrome P450 enzyme family ,Solute carrier family ,Pig ,Transcriptomics
【DOI】doi.org/10.1016/j.ecoenv.2021.111910
【影響因子(IF)】6.2
【出版期刊】《Ecotoxicology and Environmental Safety 》
【產(chǎn)品原文引用】
Serum isolated from anterior vena cava blood was used to determine alanine transaminase (ALT) and aspartate transaminase (AST) activities by Microplate assay kits (Shanghai Hengyuan Bioengineering Co., Ltd., China) according to manufacturer’s protocol. Serum ALT and AST levels were determined by double antibody sandwich method. First, the purified ALT or AST antibody of pigs was coated with the microporous plate, and then serum was added to the micropore to form the antibodyantigen-elisa antibody complex. After thorough washing, substrate TMB was added for color development. The OD values were measured by an enzyme-labeled instrument at the wavelength of 450 nm. The concentration of ALT or AST in the sample was calculated by the standard curve.
相關(guān)產(chǎn)品
免責(zé)聲明
- 凡本網(wǎng)注明“來源：化工儀器網(wǎng)”的所有作品，均為浙江興旺寶明通網(wǎng)絡(luò)有限公司-化工儀器網(wǎng)合法擁有版權(quán)或有權(quán)使用的作品，未經(jīng)本網(wǎng)授權(quán)不得轉(zhuǎn)載、摘編或利用其它方式使用上述作品。已經(jīng)本網(wǎng)授權(quán)使用作品的，應(yīng)在授權(quán)范圍內(nèi)使用，并注明“來源：化工儀器網(wǎng)”。違反上述聲明者，本網(wǎng)將追究其相關(guān)法律責(zé)任。
- 本網(wǎng)轉(zhuǎn)載并注明自其他來源（非化工儀器網(wǎng)）的作品，目的在于傳遞更多信息，并不代表本網(wǎng)贊同其觀點(diǎn)和對其真實(shí)性負(fù)責(zé)，不承擔(dān)此類作品侵權(quán)行為的直接責(zé)任及連帶責(zé)任。其他媒體、網(wǎng)站或個(gè)人從本網(wǎng)轉(zhuǎn)載時(shí)，必須保留本網(wǎng)注明的作品第一來源，并自負(fù)版權(quán)等法律責(zé)任。
- 如涉及作品內(nèi)容、版權(quán)等問題，請?jiān)谧髌钒l(fā)表之日起一周內(nèi)與本網(wǎng)聯(lián)系，否則視為放棄相關(guān)權(quán)利。
采購中心