—、多色流式分析的福音

由于熒光光譜的重疊現(xiàn)象，在進(jìn)行流式多色分析時(shí)，必須設(shè)置單染管進(jìn)行熒光補(bǔ)償。OneComp /UltraComp eBeads 微球大小和淋巴細(xì)胞相當(dāng)，可連接各種熒光標(biāo)記的抗體來(lái)調(diào)節(jié)補(bǔ)償。

每滴微球中都含有兩群：一群是可以結(jié)合抗體的陽(yáng)性群，一群是不會(huì)與抗體反應(yīng)的陰性群。

優(yōu)點(diǎn)：

★ 使用方便，一滴即可。
★ 與所有小鼠、大鼠和倉(cāng)鼠來(lái)源的抗體反應(yīng)，要求抗體來(lái)源是Kappa鏈。
★ 適用于各種激發(fā)光---適用于488 nm 藍(lán)光，532 nm 綠光，561 nm 黃光，633-635 nm 紅光，UltraComp eBeads尤其適用紫外 (355 nm) 或者 紫色 (405 nm) 激光器。

Comparison of BD CompBead and BD CompBead Plus particles on different sample types

A. H9 human embryonic stem cells (WiCell, Madison, WI), and BD CompBead (Cat. No. 552843) and BD CompBead Plus particles (Cat. No. 560497) were stained with PE mouse anti–SSEA-4 (Cat. No. 560128) and analyzed on a flow cytometer. Both unstained and stained hESCs are shown in blue, negative and stained BD CompBead particles in green, and negative and stained BD CompBead Plus particles in red. Flow cytometry was performed on a BD™ LSR II flow cytometry system.

B. Lysed whole blood (LWB), BD CompBead particles, and BD CompBead Plus particles were stained with PE mouse anti-human CD3 (Cat. No. 555333) and analyzed on a flow cytometer. Stained LWB is shown in blue, negative and stained BD CompBead particles in green, and negative and stained BD CompBead Plus particles in red. Flow cytometry was performed on a BD LSR II flow cytometry system

產(chǎn)品訂購(gòu)信息：

1、CompBead Compensation Particles通用補(bǔ)償微球

2、CompBead Plus Particles 補(bǔ)償大微球，適用于larger cell types, for example, human embryonic stem cells (hESCs)