[ TEST PRINCIPLE ]

The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP26. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MMP26. Next, Avidin conjugated to Horseradish Peroxidase （HRP） is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MMP26, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MMP26 in the samples is then determined by comparing

the O.D. of the samples to the standard curve.

[ REAGENTS AND MATERIALS PROVIDED ]

Reagents                                                                  Quantity

Pre-coated, ready to use 96-well strip plate            1

Plate sealer for 96 wells                                             4

Standard （lyophilized）                                                  2

Standard Diluent                                                       1×20mL

Detection Reagent A （green）                                  1×120μL

Assay Diluent A （2 × concentrate）                          1×6mL

Detection Reagent B （red）                                       1×120μL

Assay Diluent B （2 × concentrate）                         1×6mL

TMB Substrate                                                           1×9mL

Stop Solution                                                             1×6mL

Wash Buffer （30 × concentrate）                             1×20mL

Instruction manual                                                      1

 

[ STORAGE OF THE KITS ]

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20oC upon receipt while the others should be at 4 oC.

2. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

Note:

It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.

 

Note:

1. Samples to be used within 5 days may be stored at 4oC, otherwise samples must be stored at -20oC （≤1month） or -80oC （≤2 months） to avoid loss of bioactivity and contamination.

2. Sample hemolysis will influence the result, so hemolytic specimen can not be detected.

3. When performing the assay, bring samples to room temperature.