小鼠生長分化因子1蛋白使用說明書

[ DESCRIPTION ]

Protein Names: Growth Differentiation Factor 1
Synonyms: GDF1                    
Species: Mouse
Size: 10µg
Source: Escherichia coli-derived
Subcellular Location: Secreted.
[ PROPERTIES ]
Residues: Cys230~Arg357 （Accession # P20863）, with N-terminal His-Tag.
Grade & Purity: >93%, 17 kDa as determined by SDS-PAGE reducing conditions.
Formulation: Supplied as lyophilized form in PBS, pH 7.4, containing 0.01% Sarcosyl, 5% sucrose.
Endotoxin Level: <1.0 EU per 1μg （determined by the LAL method）.
Applications: SDS-PAGE; WB; ELISA; IP.
（May be suitable for use in other assays to be determined by the end user.）
Predicted Molecular Mass: 15.6 kDa
Predicted isoelectric point: 8.26
[ PREPARATION ]
Reconstitute in sterile PBS, pH7.2- pH7.4.

[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
         Store at 2-8oC for one month.
         Aliquot and store at -80oC for 12 months .
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. （Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.） The loss of this protein is less than 5% within the expiration date under appropriate storage condition.     
[ SEQUENCES ]
The target protein is fused with N-terminal His-tag, its sequence is listed below.
MGHHHHHHSG SEF-CPLPRLR RHTEPRVEVG PVGTCRTRRL HVSFREVGWH RWVIAPRGFL ANFCQGTCAL PETLRGPGGP PALNHAVLRA LMHAAAPTPG AGSPCCVPER LSPISVLFFD NSDNVVLRHY EDMVVDECGC R
 [ REFERENCES ]
1. Lee, S. J. （1990）. Mol. Endocrinol. 4: 1034-1040.
2. Lee, S. J. （1991）. Proceedings of the National Academy of Sciences. 88（10）: 4250.
3. Gerhard, D. S., L. Wagner, et al. （2004）. Genome research. 14（10b）: 2121.