Used in more labs than any other detergent-compatible protein assay.

The Thermo Scientific Pierce BCA Protein Assay remains one of the most popular protein assay methods worldwide. The detergent-compatible method provides for accurate determination of protein concentration with most sample types encountered in protein research laboratories. The Pierce BCA Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

Highlights:

• Colorimetric method; read at 562 nm
• Compatible with most ionic and nonionic detergents
• Faster and easier than the Lowry method
• Working reagent stable for 24 hours
• Linear working range for BSA from 20-2,000 µg/ml
• Minimum detection level of 5 µg/ml with the enhanced protocol
• Adaptable to microplates
• Less protein-to-protein variation than dye-binding methods

BCA Protein Assay Applications:

• Studying protein:protein interactions
• Measuring column fractions after affinity chromatography
• Estimating percent recovery of membrane proteins from cell extracts
• High-throughput screening of fusion proteins