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CYT-209

重組人白介素-2 （rHuIL-2）

10μg/50μg/1mg

Prospec

Description :
	Recombinant Human IL-2 produced in E.Coli is a single, non-glycosylated mutein （variant form） of human IL-2 polypeptide chain containing 134 amino acids and having a molecular mass of 15517 Dalton. Our rHuIL-2 has an Ser substitute for Cysteine at position 126. The rHuIL-2 is purified by proprietary chromatographic techniques.
	Physical Appearance:
	Sterile Filtered White lyophilized （freeze-dried） powder.
	Formulation:
	The protein （1.1mg/ml） was lyophilized after extensive dialysis against 0.17mg sodium monobasic & 0.89mg dibasic sodium phosphate buffer to a pH=7.5.
	Solubility:
	It is recommended to reconstitute the lyophilized IL-2 in sterile 20mM acetic acid （AcOH） not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
	Stability:
	Lyophilized rHuIL-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuIL-2 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.
	Purity:
	Greater than 98.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
	Amino acid sequence:
	The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser.
	Dimers and aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
	Biological Activity:
	ProSpec's rHuIL-2 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is less then 0.0645 ng/ml, corresponding to a Specific Activity of 16.9 MIU/mg.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of HuIL-2.
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.614 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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