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ExoView外泌體全面表征試劑盒
外泌體計數(shù)、粒徑、蛋白表達(dá)、蛋白共定位次完成
檢測樣本類型 | 對細(xì)胞培養(yǎng)上清、血漿、血清、尿液、腦脊液、唾液等生物樣本中的外泌體直接進(jìn)行分析 |
捕獲抗體種類 | anti-CD81, anti-CD9, anti-CD63, 同型IgG對照；可自定義 |
單次上樣體積 | 35 µl稀釋樣本 |
重復(fù)檢測數(shù)目 | 3復(fù)孔 |
熒光抗體種類 | CD9（Blue）/ CD81（Green）/ CD63（Red） |
實驗原理
① 35 μL外泌體樣品滴加在芯片上孵育；
② 預(yù)包被的抗體異結(jié)合外泌體表面蛋白以捕獲外泌體；
③ 再使用熒光抗體異性標(biāo)記需要表征的標(biāo)記物；
④ 后用ExoView R100檢測外泌體粒徑、計數(shù)、蛋白表達(dá)（CD9，CD81，CD63等）及共定位。
檢測流程
產(chǎn)品類別
| 產(chǎn)品貨號 | 產(chǎn)品名稱 |
| EV-TETRA-C | 人外泌體檢測試劑盒 |
| EV-TETRA-P | 人血漿外泌體檢測試劑盒 |
| EV-TETRA-M2 | 鼠外泌體檢測試劑盒 |
| EV-TETRA-C-CAR | 人外泌體內(nèi)容物檢測試劑盒 |
| EV-TETRA-P-CAR | 人血漿外泌體內(nèi)容物檢測試劑盒 |
| EV-TC-FLEX | 自由捕獲人外泌體檢測試劑盒 |
| EV-TP-FLEX | 自由捕獲人血漿外泌體檢測試劑盒 |
| EV-TC-FLEX-CAR | 自由捕獲人外泌體內(nèi)容物檢測試劑盒 |
| EV-TP-FLEX-CAR | 自由捕獲人血漿外泌體內(nèi)容物檢測試劑盒 |
| EV-TM-FLEX | 自由捕獲鼠外泌體檢測試劑盒 |
| EV-TM-FLEX-CAR | 自由捕獲鼠外泌體內(nèi)容物檢測試劑盒 |
| EV-FLEX-2 | 自由捕獲外泌體檢測試劑盒 |
| EV-FLEX-2 -CAR | 自由捕獲外泌體內(nèi)容物檢測試劑盒 |
| EV-CTETRA-1/2/3 | 人外泌體檢測試劑盒+1/2/3個自定義捕獲抗體 |
| EV-CTETRA-1/2/3-CAR | 人外泌體內(nèi)容物檢測試劑盒+1/2/3個自定義捕獲抗體 |
| EV-CUST-1/2/3/4/5/6 | 自定義1/2/3/4/5/6抗體捕獲外泌體檢測試劑盒 |
| EV-CUST-1/2/3/4/5/6-CAR | 自定義1/2/3/4/5/6抗體捕獲外泌體內(nèi)容物檢測試劑盒 |
試劑盒點
異性捕獲 芯片上可包被多達(dá)6種捕獲抗體，異性捕獲含定蛋白標(biāo)記物的外泌體。
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對陽性外泌體計數(shù) 芯片捕獲外泌體后，可通過SP-IRIS技術(shù)直接檢測樣品中外泌體的數(shù)量。
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單個外泌體蛋白共定位分析 檢測每個外泌體的熒光信號并進(jìn)行統(tǒng)計，可獲得熒光共定位信息，用于分析樣品中不同表型外泌體的比例（如右圖所示）。
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無需純化
使用抗體捕獲模式，防止樣品中雜質(zhì)影響結(jié)果，可直接檢測血液、尿液和細(xì)胞培養(yǎng)液中的外泌體，未純化樣品的測量結(jié)果與純化后基本致（如右圖所示）。
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粒徑分辨率高
高精度SP-IRIS技術(shù)，可檢測≥50 nm的外泌體，測量結(jié)果與電子顯微鏡檢測結(jié)果基本致，并統(tǒng)計生成外泌體的粒徑分布結(jié)果（如右圖所示）。
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可檢測外泌體內(nèi)容物
試劑盒配套相應(yīng)的穿膜劑，可穿透外泌體并對外泌體內(nèi)容物進(jìn)行染色并檢測，未穿膜時只能檢測到跨膜蛋白CD9的熒光信號，穿膜后即可檢測到外泌體內(nèi)容物Syntenin的表達(dá)（如右圖所示）。
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測試數(shù)據(jù)
外泌體熒光數(shù)量統(tǒng)計
外泌體粒徑檢測
外泌體內(nèi)容物檢測
熒光強(qiáng)度與粒徑關(guān)系
熒光共定位分析
發(fā)表文章
• Andras Saftics.(2021) Data evaluation for surface-sensitive label-free methods to obtain real-time kinetic and structural information of thin films: A practical review with related software packages. Advances in Colloid and Interface Science.
• Kyoung-Won Ko.(2021) Integrated Bioactive Scaffold with Polydeoxyribonucleotide and Stem-Cell-Derived Extracellular Vesicles for Kidney Regeneration. ACS Nano.
• Tanina Arab. (2021) Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single‐particle analysis platforms. Journal of Extracellular Vesicles.
• Niaz Z.Khan.(2021) Spinal cord injury alters microRNA and CD81+ exosome levels in plasma extracellular nanoparticles with neuroinflammatory potential. Brain, Behavior, and Immunity.
• Dario Brambilla. (2021) EV Separation: Release of Intact Extracellular Vesicles Immunocaptured on Magnetic Particles. Analytical Chemistry.
• Enkhtuya Radna. (2021) Extracellular vesicle mediated feto-maternal HMGB1 signaling induces preterm birth. Lab on a Chip.
• Li, M., Soder. (2021) WJMSC‐derived small extracellular vesicle enhance T cell suppression through PD‐L1. Journal of Extracellular Vesicles.
• Crescitelli, R. (2021) Isolation and characterization of extracellular vesicle subpopulations from tissues. Nature protocols.
• Berger, A. (2021). Local administration of stem cell-derived extracellular vesicles in a thermoresponsive hydrogel promotes a pro-healing effect in a rat model of colo-cutaneous post-surgical fistula. Nanoscale.
• Vidal, M. (2020) Exosomes and GPI-anchored proteins: Judicious pairs for investigating biomarkers from body fluids. Advanced drug delivery reviews.
• K Cho, H Kook.(2020)Study of immune-tolerized cell lines and extracellular vesicles inductive environment promoting continuous expression and secretion of HLA-G from semiallograft immune tolerance during pregnancy. Journal of Extracellular Vesicles.
• Maximillian A. Rogers.(2020)Annexin A1–dependent tethering promotes extracellular vesicle aggregation revealed with single–extracellular vesicle analysis. Cell Biology.
• Annette M. Marleau.(2020)Targeting tumor-derived exosomes using a lectin affinity hemofiltration device. Cancer Research.
• Alessandro Gori.(2020)Membrane-Binding Peptides for Extracellular Vesicles On-Chip Analysis. Journal of Extracellular Vesicles.
• Rossella Crescitelli.(2020)Subpopulations of extracellular vesicles from human metastatic melanoma tissue identified by quantitative proteomics after optimized isolation. Journal of Extracellular Vesicles.
• Maria S. Panagopoulou.(2020) Phenotypic analysis of extracellular vesicles: a review on the applications of fluorescence. Journal of Extracellular Vesicles.
• WeiYan.(2020) Immune Cell-Derived Exosomes in the Cancer-Immunity Cycle. Trends in Cancer.
• Daniel Bachurski. (2019) Small RNA Sequencing across Diverse Biofluids Identifies Optimal Methods for exRNA Isolation. Cell.
用戶單位
