19
本產(chǎn)品僅用于科研，不用于臨床
MOC2細胞系凍存或復蘇、傳代或原代、進口及國產(chǎn)提供，公司供應的細胞生長健康，活性優(yōu)良，現(xiàn)公司能提供給客戶的細胞株及細胞系達三千多種，涵蓋細胞所有研究領域，國內外科研單位，我們供貨。質量保證，可定制包裝規(guī)格，發(fā)貨快速，同各個相關科研機構建立了良好的合作關系，歡迎您、訂購優(yōu)勢的產(chǎn)品!
FisherScientific:
T150Flasks : 07-200-64
T75 Flasks : 10-126-37
Cryovials : 03-374-059
45um filters: : 09-754-21
05% Trypsin : sh30236.01
25% Trypsin : sh30042.01
Indolent Lines – MOC1, 22
Aggressive Lines – MOC2
Thawing cell lines
1. Add 21ml IMDM MOC line media to a T150 before thawing (or 10ml to a T75 if wanting to thaw into aT75)
2. Remove cryovial from liquid nitrogen, spray vial with 70% alcohol to clean it.
3. Hold bottom-half of cryovial in 37Cwaterbath (without letting lid touch water, to avoid contamination) until there is a small chunk of ice left floating.
4. Spray cryovial again with ETOH and place in hood. Pipette 1ml of media to the 1ml of cells and add these 2ml to the T150 that already contains media (to make 22ml total for one T150).
5. Take some media already in T150 flask and rinse the cryovialand plate this to ensure you have all the residual cells left in the cryovial.
MOC2細胞系保存注意事項：
低溫、避光、干燥陰涼處封閉貯存，嚴禁與有毒、有害物品混放、混運。本品為非危險品，可按一般化學品運輸，輕搬動輕放，防止日曬、雨淋。
