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Detroit 562細胞， 人咽頭癌胸水轉(zhuǎn)移細胞
| Organism | Homo sapiens, human |
|---|---|
| Tissue | pharynx: derived from metastatic site: pleural effusion |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1 |
| Disease | pharyngeal carcinoma |
| Age | ***** |
| Gender | female |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
Karyotype Modal number = 64; range = 58 to128
A large subterminal marker chromosome, arm ratio 3:4, is found in 94% of the cells karyotyped. Five to 6 minute chromosomes are present in each cell. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines Clinical Data
Detroit 562細胞， 人咽頭癌胸水轉(zhuǎn)移細胞
Caucasian
female
Genes Expressed
keratin
Virus Susceptibility Human poliovirus 1
Vesicular stomatitis virus
Comments
The cells are positive for keratin by immunoperoxidase staining.
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation | Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage Temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: Air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C CCL-138 Detroit 562 人咽頭癌胸水轉(zhuǎn)移細胞 |
