欧美……一区二区三区,欧美日韩亚洲另类视频,亚洲国产欧美日韩中字,日本一区二区三区dvd视频在线

上海復(fù)祥生物科技有限公司

當(dāng)前位置:上海復(fù)祥生物科技有限公司>>細(xì)胞庫>>人正常細(xì)胞>>CRL-2234SNU-449細(xì)胞, 人肝癌細(xì)胞

SNU-449細(xì)胞, 人肝癌細(xì)胞

參   考   價: ¥ 1

訂  貨  量: ≥1 件

具體成交價以合同協(xié)議為準(zhǔn)

產(chǎn)品型號:CRL-2234

品       牌:其他品牌

廠商性質(zhì):生產(chǎn)商

所  在  地:上海市

更新時間:2024-11-16 17:02:15瀏覽次數(shù):1863

聯(lián)系我時,請告知來自 化工儀器網(wǎng)
供貨周期 一周 規(guī)格 T25
貨號 SNU-449細(xì)胞 應(yīng)用領(lǐng)域 醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥
主要用途 科學(xué)研究
SNU-449細(xì)胞, 人肝癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞,細(xì)胞庫管理規(guī)范,提供 *培養(yǎng)條件

SNU-449細(xì)胞, 人肝癌細(xì)胞(ATCC® CRL-2234)

Permits and Restrictions

View Restrictions

OrganismHomo sapiens, human
Tissue

liver

Product Formatfrozen
Morphologyepithelial; diffusely spreading cells
Culture Propertiesadherent
Biosafety Level2  [Cells contain Hepatitis B virus]
Diseasegrade II-III/IV,hepatocellular carcinoma
Age52 years
Gendermale
EthnicityAsian
Storage Conditionsliquid nitrogen vapor phase
Karyotypeaneuploid; modal number = 57
Derivation

SNU-449 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient prior to cytotoxic therapy.

Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum.
Clinical Data

52 years

Asian

male

SNU-449細(xì)胞, 人肝癌細(xì)胞

Comments

Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed.

Grossly, the original tumor was single nodular with perinodular extensions. Histologically, it was predominantly compact and minor trabecular type.

The cultured cells contain a single or double nucleus.

Complete Growth MediumThe base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
SubculturingProtocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:5 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days


Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37°C























HK-2 人腎近曲小管上皮細(xì)胞

CRL-2190 HK-2 人腎近曲小管上皮細(xì)胞,

人肺成纖維細(xì)胞

CCL-153 HFL1 人肺成纖維細(xì)胞,原代細(xì)胞

人肝星形細(xì)胞

LX-2 人肝星形細(xì)胞,$r$nATCC 細(xì)胞|細(xì)

會員登錄

X

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個,單個標(biāo)簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復(fù)您~

以上信息由企業(yè)自行提供,信息內(nèi)容的真實性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),化工儀器網(wǎng)對此不承擔(dān)任何保證責(zé)任。

溫馨提示:為規(guī)避購買風(fēng)險,建議您在購買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

撥打電話
在線留言
老肥妇色一区二区三区| 一级黄色按摩风骚的少妇| 自拍偷拍第一综合区图| 免费午夜福利视频在线| 日韩五十路六十路熟女av| 巨人福利视频网址导航| 免费精品国产一区二区三区| 成av免费大片黄在线观看| 天堂欧美日韩在线观看| 中文字幕乱码六在线视频| 久久久精品免费视频看看| 在线日韩精品中文字幕| 午夜免费看夫妻生活片| 欧美激情在线观看播放| 日韩三级伦理片色呦呦中文字幕| 欧美精品一区视频在线高清| 欧美日韩在线观看二区| 国产性感美女在线播放| 五十路六十路熟女av| 精品女厕一区二区三区| 欧美精品一区二区三区 高清| 日本一区二区三区四区在线视频| 欧美日韩国产中文字幕| 色综合91精品中文字幕| 成人18禁在线免费观看| 99久久精品国产婷婷| 欧美网页一区在线激情观看| 激情五月天欧美 国产| 免费午夜福利视频在线| 不卡日韩欧美在线视频| 人妻少妇视频一区二区| 中文字幕国产精品久久久| 香蕉人多人在线观看久草| 久久国内一区人妻日韩| 玩弄放荡人妻系列一区二区| 京香一区二区三区中文字幕| 国产精品久久久精品久久| 伊人色啪啪天天综合婷婷| 成人在线av一区二区| 亚洲精品免费观看av| 国产私人尤物福利视频|