Invitrogen 28025-013 M-MLV Reverse Transcriptase 簡介:
產(chǎn)品名稱:M-MLV Reverse Transcriptase
貨 號:28025-013
規(guī) 格:40,000U
品 牌:Invitrogen
國產(chǎn)/進(jìn)口:進(jìn) 口
儲存溫度:-20°C
特惠價格:致 電
庫存狀態(tài):現(xiàn) 貨
銷售商:上?;鄯f生物科技有限公司
慧穎生物專業(yè)供應(yīng)Invitrogen 28025-013 M-MLV Reverse Transcriptase,常備現(xiàn)貨,*。主營Life Invitrogen產(chǎn)品,囊括TRlzol ?Reagent、TRIZOL LS Reagent 、B-27添加劑、凝膠填料、常規(guī)化學(xué)試劑、培養(yǎng)基及原料試劑、各類胎牛血清、馬血清等,如有需要,詳詢。
Invitrogen 28025-013 M-MLV Reverse Transcriptase描述:
MMLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RT) is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. Compared to AMV RT, M-MLV RT lacks DNA endonuclease activity and has a lower RNase H activity.
Thermostability – Optimal activity at 37°C
Size of cDNA – MMLV can be used to synthesize first-strand cDNA up to 7 kb
Applications – Synthesis of first-strand cDNA (1), primer extension (2), sequencing dsDNA (3), cDNA libraries, and RT-PCR (4)
Source: Purified from E. coli expressing the pol gene of M-MLV on a plasmid (5).
Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.
Unit Definition: One unit of M-MLV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)?oligo(dT)12-18 as template?primer (6).
Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 μl for 10 min. at 37°C (6).
Invitrogen 28025-013 M-MLV Reverse Transcriptase同類產(chǎn)品主要有:
貨號 | 名稱 | 規(guī)格 |
14750100 | BLOCKIT ALEXA FLUOR RED OLIGO | 20 UM- 250UL |
K494500 | BLOCK IT U6 ENTRY VECTOR KIT | 20 RXNS |
15630080 | HEPES | 10ml |
NP0060 | NUPAGE MES BIS-TRIS BFR KIT | COMBO |
NP0321BOX | NuPAGE? Novex 4-12% Bis-Tris Gel 1.0 mm, 10 Well | 10 gels?box |
12355036 | PFX50 DNA POL., 500RXN | 500 RXNS |
A11180 | CLONEMINER CDNA LIB KIT, VER2 | 5 RXN KIT |
L150201 | GENERACER SUPERSCRPT TA | 5 CDNA- 50 PCR RXNS |
11791020 | LR CLONASE II ENZYME MIX | 20 REACTIONS |
10928-034 | 含有Platinum? Taq DNA聚合酶的SuperScriptTM II一步法RT-PCR系統(tǒng) | 25 rxns |
10928-042 | 100 rxns | |
11904-018 | 用于RT-PCR的SuperScriptTM II*鏈合成系統(tǒng) | 50 rxns |
18080-200 | SuperScriptTM III CellsDirect cDNA合成試劑盒 | 25 rxns |
18080-300 | 100 rxns | |
18080-400 | SuperScriptTM III*鏈合成SuperMix | 50 rxns |
18068-015 | DNase I擴(kuò)增級 | 100U(1U/ul) |
48190-011 | 隨機(jī)引物 | 9 units |
K210005 | PURELINK PLASMID MIDI 50 | 50 REACTIONS |
S7563 | SYBR? Green I核酸凝膠染料 | 500ul |
S7567 | 1ml | |
S7564 | SYBR? Green II RNA凝膠染料 | 500ul |
S7568 | 1ml | |
11811031 | GENETICIN | 5G |
10459014 | DMRIE-C REAGENT | 1ml |
12309019 | OPTI PRO SFM | 1000ml |
11514015 | PLUS REAGENT | 0.85 ML |
12651014 | FREESTYLE CHO EXPRESSION MEDIU | 1000ML |
12338018 | FREESTYLE 293 EXPRESSION MED | 1000ML |
12347019 | 293FECTIN | 1ml |
16447100 | FreeStyle MAX | 1 ml |
R25001 | ZEOCIN | 1g |
15224-017 | T4 DNA 連接酶 | 100U(1U/ul) |
15224-025 | 500U(1U/ul) | |
15224-090 | 4x500U(1U/ul) | |
15224-041 | 250U(5U/ul) | |
18047-019 | DNase I | 20,000U |
18068-015 | DNase 擴(kuò)增級 | 100U |
18021-014 | 核糖核酸酶H | 30U(2U/ul) |
18021-071 | 4x30U(2U/ul) | |
18162-016 | DNA聚合酶I/DNase I | 250U |
18005-017 | T4 DNA 聚合酶 | 50U(5U/ul) |
18005-025 | 250U(5U/ul) | |
25530-015 | 蛋白酶K | 100mg |
25530-031 | 1g | |
18374-058 | 5`RACE系統(tǒng), | 10 rxns |
18373-019 | 3`RACE系統(tǒng), | 20 rxns |
MPK10096 | Neon? Transfection System 100 μL Kit | |
K280020 | ZERO BLUNT TOPO PCRCLON KIT | 20 RXN |
18012021 | LARGE FRAGMENT | 100 UN |
18021014 | RNASE H (E.COLI) | 30 UN |
4311816 | AmpliTaq Gold? DNA Polymerase, 1000 units with Gold Buffer and MgCl2 solution | 1000 un |
18092015 | CDNA SIZE FRAC COLUMNS | 3 COLUMNS |
450512 | BLOCK-IT H1 RNAI VECTOR KIT | 20 RXNS |
10511012 | 1 kb DNA Extension Ladder | 100 UG |
Invitrogen產(chǎn)品種類較多,本展臺無法一一體現(xiàn),以上是部分產(chǎn)品,若有需要的產(chǎn)品,請及時與我們?nèi)〉茫稍儭?/span>
電 話:/
傳 真:
郵 箱:1242635944
網(wǎng) 址:http://www.biohysw。。com/