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產(chǎn)品名稱：Invitrogen 450008 TOPO XL PCR CLONING KIT
貨 號(hào)：450008
規(guī) 格：10 RXN
分 類： PCR 克隆載體
品 牌：Invitrogen
國產(chǎn)/進(jìn)口：進(jìn) 口
儲(chǔ)存溫度：-20°C
特惠價(jià)格：致 電
庫存狀態(tài)：現(xiàn) 貨
銷售商：上?；鄯f生物科技有限公司
慧穎生物專業(yè)供應(yīng)Invitrogen 450008 TOPO XL PCR CLONING KIT，常備現(xiàn)貨，*。主營Life Invitrogen產(chǎn)品，囊括TRlzol ?Reagent、TRIZOL LS Reagent 、B-27添加劑、凝膠填料、常規(guī)化學(xué)試劑、培養(yǎng)基及原料試劑、各類胎牛血清、馬血清等，如有需要，詳詢。
描述：
The TOPO? XL PCR Cloning Kit combines a unique vector and novel protocol for easy, efficient cloning of long (3-10 kb) PCR products. The kit uses the linearized and topoisomerase I-activated pCR?-XL-TOPO? vector. Topoisomerase I activation allows PCR products to be ligated in just 5 minutes on your bench top and typically results in >80% recombinants. The pCR?-XL-TOPO? vector includes:
? ccdB gene for positive selection
? EcoR I and Nsi I sites flanking the PCR product insertion site for easy excision of inserts
? Kanamycin and Zeocin? resistance genes for your choice of selection in E. coli
? T7 promoter/priming site for in vitro RNA transcription and sequencing
? M13 forward and reverse primer sites for sequencing
Novel Protocol
The TOPO? XL PCR Cloning Kit protocol includes S.N.A.P.? gel purification which employs crystal violet for improved efficiency of long PCR cloning. This eliminates the need for ethidium bromide and exposure to UV light, which can damage long PCR products and reduce the number of recombinants.
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