會(huì)員.png)
13
MC3T3-E1 Subclone 14細(xì)胞 全國統(tǒng)一價(jià)格，全國各地均可發(fā)貨，遠(yuǎn)到香港、澳門、福建、新疆、內(nèi)蒙古等地。
產(chǎn)品名稱：MC3T3-E1 Subclone 14
中文名稱：小鼠顱頂前骨細(xì)胞亞克隆14
來源：美國ATCC
生長狀態(tài)：貼壁生長|懸浮生長|半貼壁半懸浮生長
細(xì)胞形態(tài)：上皮樣|成纖維樣|圓形|不規(guī)則
培養(yǎng)條件：DMEM|1640|MEM|IMDM+10% FBS
細(xì)胞規(guī)格：5-10×105細(xì)胞量
特征特性：MC3T3-E1 Subclone 14細(xì)胞 從克隆的但是表型各異的MC3T3-E1細(xì)胞系中分離出一系列亞克隆。從含抗壞血酸培養(yǎng)基生長的成骨細(xì)胞中選擇高或低成骨細(xì)胞分化、礦化的亞克隆。MC3T3亞克隆4（ATCC CRL-2593）和MC3T3亞克隆14（ATCC CRL-2594）在抗壞血酸和3到4mM無機(jī)磷酸鹽中生長表現(xiàn)出高水平的成骨細(xì)胞分化。它們10天后形成一個(gè)礦化良好的細(xì)胞外基質(zhì)（ECM）。MC3T3亞克隆24（ATCC CRL-2595)和MC3T3亞克隆30(ATCC CRL-2596)在抗壞血酸中生長表現(xiàn)出很差的成骨細(xì)胞分化。不形成ECM，可以作為亞克隆4和14的陰性對(duì)照。礦化的亞克隆選擇的表達(dá)作為成骨細(xì)胞標(biāo)記的mRNA，及唾液酸糖蛋白(BSP)，骨鈣素(OCN)，和甲狀旁腺激素/甲狀旁腺激素相關(guān)蛋白受體的mRNA。高或者低的分化潛能的亞克隆在培養(yǎng)中生產(chǎn)出相似數(shù)量的膠原質(zhì)，表達(dá)可比較的基本水平的mRNA編碼Osf2/Cbfa1，一種成骨細(xì)胞相關(guān)轉(zhuǎn)錄因子。植入免疫缺陷小鼠以后，高分化性的亞克隆形成與骨類似的形成小骨的編織骨，低分化細(xì)胞只是產(chǎn)生纖維組織。這些細(xì)胞系是研究體外成骨細(xì)胞分化的好模型，尤其是ECM信號(hào)。它們和原代培養(yǎng)顱頂成骨細(xì)胞的行為類似。
庫存狀態(tài)：現(xiàn)貨
運(yùn)輸方式：運(yùn)輸方式分為新鮮運(yùn)輸和凍存管運(yùn)輸兩種運(yùn)輸方式
貨期：新鮮運(yùn)輸需要1-2周，凍存管運(yùn)輸?shù)男枰?-3天
慧穎細(xì)胞庫提供的所有細(xì)胞僅供科研使用
原代細(xì)胞的培養(yǎng)是指直接從機(jī)體取下細(xì)胞、組織和器官后立即進(jìn)行培 養(yǎng)；因此，較為嚴(yán)格地說是指成功傳代之前的培養(yǎng)，此時(shí)的細(xì)胞保持原有細(xì)胞的基本性質(zhì)，如果是正常細(xì)胞，仍然保留二倍體數(shù)。不同的原代細(xì)胞傳代次數(shù)不同，具體需咨詢客服人員或者自行查閱 相關(guān)資料。
細(xì)胞株是通過選擇法或克隆形成法從原代培養(yǎng)物或細(xì)胞系中獲得的具有特殊性質(zhì)或標(biāo)志的培 養(yǎng)細(xì)胞。從培養(yǎng)代數(shù)來講，理論上可培養(yǎng)到40-50代。
客戶應(yīng)具有一定細(xì)胞培養(yǎng)知識(shí)與經(jīng)驗(yàn)，并具備基本培養(yǎng)條件（細(xì)胞培養(yǎng)實(shí)驗(yàn)室，無菌操作臺(tái)、CO2培養(yǎng)箱，可拍照倒置顯微鏡等），并按說明書要求準(zhǔn)備好相應(yīng)的無菌的培養(yǎng)基、血清、雙抗、細(xì)胞培養(yǎng)瓶、培養(yǎng)板等。如果因客戶缺少基本培養(yǎng)經(jīng)驗(yàn)和培養(yǎng)條件而導(dǎo)致細(xì)胞死亡或污染，不在我司售后責(zé)任之內(nèi)。
相關(guān) 熱賣產(chǎn)品：
骨、關(guān)節(jié)、軟組織和皮膚系統(tǒng)Bones, joints,soft tissue and skin system
BJ細(xì)胞，人原代成纖維細(xì)胞 5 × 105 1-2weeks
HSF細(xì)胞， 人皮膚成纖維細(xì)胞 5 × 105 1-2weeks
FLS細(xì)胞， 成纖維樣滑膜細(xì)胞 5 × 105 1-2weeks
A-431細(xì)胞，人皮膚鱗癌細(xì)胞 5 × 105 1-2weeks
SK-MEL-1 細(xì)胞，人皮膚黑色素瘤細(xì)胞 5 × 105 1-2weeks
A875細(xì)胞，人黑色素瘤細(xì)胞 5 × 105 1-2weeks
A-375細(xì)胞， 人惡性黑色素瘤細(xì)胞 5 × 105 1-2weeks
HaCaT細(xì)胞， 人永生化表皮細(xì)胞 5 × 105 1-2weeks
hFOB1.1細(xì)胞，人永生化成骨細(xì)胞 5 × 105 1-2weeks
hFOB 1.19細(xì)胞，SV40轉(zhuǎn)染人成骨細(xì)胞 5 × 105 1-2weeks
HT-1080細(xì)胞，人纖維肉瘤細(xì)胞 5 × 105 1-2weeks
MG-63細(xì)胞，人成骨肉瘤細(xì)胞 5 × 105 1-2weeks
Saos-2細(xì)胞， 人成骨肉瘤細(xì)胞 5 × 105 1-2weeks
TE671 subline No.2 人橫紋肌肉瘤細(xì)胞 5 × 105 1-2weeks
U266細(xì)胞，人骨髓瘤細(xì)胞 5 × 105 1-2weeks
HOS細(xì)胞，人骨肉瘤細(xì)胞 5 × 105 1-2weeks
U-2 OS細(xì)胞，人骨肉瘤細(xì)胞 5 × 105 1-2weeks
h-BMSC細(xì)胞，人骨髓間質(zhì)細(xì)胞 5 × 105 1-2weeks
呼吸系統(tǒng)respiratory system
16HBE細(xì)胞，人支氣管上皮樣細(xì)胞 5 × 105 1-2weeks
95-D細(xì)胞，人高轉(zhuǎn)移肺癌細(xì)胞 5 × 105 1-2weeks
H322細(xì)胞，肺癌細(xì)胞 5 × 105 1-2weeks
A549細(xì)胞，人肺癌細(xì)胞 5 × 105 1-2weeks
H1299細(xì)胞，人肺癌細(xì)胞 5 × 105 1-2weeks
PC9細(xì)胞， 人肺癌細(xì)胞 5 × 105 1-2weeks
PC14細(xì)胞， 人肺癌細(xì)胞 5 × 105 1-2weeks
SPCA-1細(xì)胞，人肺腺癌細(xì)胞 5 × 105 1-2weeks
Calu-3細(xì)胞，人肺腺癌細(xì)胞 5 × 105 1-2weeks
LTEP-a-2細(xì)胞，人肺腺癌細(xì)胞 5 × 105 1-2weeks
MRC-5細(xì)胞，人胚肺成纖維細(xì)胞 5 × 105 1-2weeks
WI-38細(xì)胞， 人胚胎肺成纖維細(xì)胞 5 × 105 1-2weeks
A549（成瘤）細(xì)胞， 人非小細(xì)胞肺癌細(xì)胞 5 × 105 1-2weeks
NCI-H358細(xì)胞，人非小細(xì)胞肺癌細(xì)胞 5 × 105 1-2weeks
NCI-H1650 細(xì)胞， 人非小細(xì)胞肺癌細(xì)胞 5 × 105 1-2weeks
NCI-H1975細(xì)胞，人非小細(xì)胞肺腺癌細(xì)胞 5 × 105 1-2weeks
NCI-N838細(xì)胞， 人非小細(xì)胞肺癌細(xì)胞 5 × 105 1-2weeks
SK-MES-1細(xì)胞， 人肺鱗癌細(xì)胞 5 × 105 1-2weeks
NCI-H226 細(xì)胞，人肺鱗癌細(xì)胞 5 × 105 1-2weeks
NCI-H520細(xì)胞，人肺鱗癌細(xì)胞 5 × 105 1-2weeks
NCI-H460細(xì)胞， 人大細(xì)胞肺癌細(xì)胞 5 × 105 1-2weeks
NCI-H661細(xì)胞， 人大細(xì)胞肺癌細(xì)胞 5 × 105 1-2weeks
泌尿系統(tǒng)urinary system
293 人胚腎細(xì)胞 5 × 105 1-2weeks
293A 人胚腎細(xì)胞 5 × 105 1-2weeks
293F 人胚腎細(xì)胞 5 × 105 1-2weeks
HEK293 人胚腎細(xì)胞 5 × 105 1-2weeks
293XL-hTLR7 人胚腎細(xì)胞 5 × 105 1-2weeks
293XL-hTLR9 人胚腎細(xì)胞 5 × 105 1-2weeks
293-mTLR5 人胚腎細(xì)胞 5 × 105 1-2weeks
293 xhTLR7 人胚腎細(xì)胞 5 × 105 1-2weeks
293 xhTLR9 人胚腎細(xì)胞 5 × 105 1-2weeks
293H 人胚腎細(xì)胞 5 × 105 1-2weeks
293FT 人胚腎細(xì)胞-用于慢病毒包裝 5 × 105 1-2weeks
293TN 人胚腎細(xì)胞--用于慢病毒包裝 5 × 105 1-2weeks
293T/17 人胚腎細(xì)胞-用于慢病毒包裝 5 × 105 1-2weeks
293LVT 人胚腎細(xì)胞--用于慢病毒包裝 5 × 105 1-2weeks
293T 人胚腎細(xì)胞（SV40T基因修飾） 5 × 105 1-2weeks
LNCaP clone FGC 人前列腺癌細(xì)胞 5 × 105 1-2weeks
DU 145 人前列腺癌細(xì)胞 5 × 105 1-2weeks
786-O 人腎透明細(xì)胞癌細(xì)胞 5 × 105 1-2weeks
Caki-1 人腎透明細(xì)胞癌皮膚轉(zhuǎn)移細(xì)胞 5 × 105 1-2weeks
G401 人腎癌Wilms細(xì)胞 5 × 105 1-2weeks
HK-2 人腎皮質(zhì)近曲小管上皮細(xì)胞 5 × 105 1-2weeks
ADPC 人雄激素依靠性前列腺癌細(xì)胞 5 × 105 1-2weeks
RWPE-1 人正常前列腺上皮細(xì)胞 5 × 105 1-2weeks
T24 人膀胱癌細(xì)胞 5 × 105 1-2weeks
EJ 人膀胱癌細(xì)胞 5 × 105 1-2weeks
5637 人膀胱癌細(xì)胞 5 × 105 1-2weeks
ScaBER 人膀胱鱗癌細(xì)胞 5 × 105 1-2weeks
內(nèi)分泌系統(tǒng)endocrine system
BT-474 人乳腺導(dǎo)管癌細(xì)胞 5 × 105 1-2weeks
435（中科：MDA-MB-435S ） 人乳腺導(dǎo)管癌 5 × 105 1-2weeks
HS-578T 人乳腺癌細(xì)胞 5 × 105 1-2weeks
MCF-7 人乳腺癌細(xì)胞 5 × 105 1-2weeks
MDA-MB-231 人乳腺癌細(xì)胞 5 × 105 1-2weeks
T47D 人乳腺癌細(xì)胞 5 × 105 1-2weeks
LLC 人乳腺癌細(xì)胞 5 × 105 1-2weeks
MCF 7 人乳腺癌細(xì)胞 5 × 105 1-2weeks
MDA-MB-231 人乳腺癌細(xì)胞 5 × 105 1-2weeks
SKBR3 人乳腺腺癌細(xì)胞 5 × 105 1-2weeks
MCF 10A 人正常乳腺上皮細(xì)胞 5 × 105 1-2weeks
SW579 人甲狀腺鱗癌細(xì)胞 5 × 105 1-2weeks
TT 人髓狀甲狀腺腫瘤細(xì)胞 5 × 105 1-2weeks
腦和神經(jīng)系統(tǒng)The brain and nervous system
A172 人膠質(zhì)母細(xì)胞瘤細(xì)胞 5 × 105 1-2weeks
BE(2)-M17 人神經(jīng)母細(xì)胞瘤細(xì)胞 5 × 105 1-2weeks
IMR-32 人神經(jīng)母細(xì)胞瘤細(xì)胞 5 × 105 1-2weeks
SH-SY5Y 人神經(jīng)母細(xì)胞瘤細(xì)胞 5 × 105 1-2weeks
SK-N-SH 人神經(jīng)母細(xì)胞瘤細(xì)胞 5 × 105 1-2weeks
SHG-44 人膠質(zhì)瘤細(xì)胞 5 × 105 1-2weeks
HS 683 人腦膠質(zhì)瘤細(xì)胞 5 × 105 1-2weeks
U-373MG 人腦神經(jīng)膠質(zhì)瘤細(xì)胞 5 × 105 1-2weeks
U251 人神經(jīng)膠質(zhì)細(xì)胞瘤細(xì)胞 5 × 105 1-2weeks
U-87 MG 人腦星形膠質(zhì)母細(xì)胞瘤細(xì)胞 5 × 105 1-2weeks
生殖系統(tǒng)reproductive system
C-33A 人宮頸癌細(xì)胞 5 × 105 1-2weeks
Ca Ski 人宮頸癌細(xì)胞 5 × 105 1-2weeks
Hela229 人宮頸癌細(xì)胞 5 × 105 1-2weeks
HeLa 人宮頸癌細(xì)胞 5 × 105 1-2weeks
人胚胎成纖維細(xì)胞 5 × 105 1-2weeks
HEC-1-B 人子宮內(nèi)膜腺癌細(xì)胞 5 × 105 1-2weeks
Ishikawa 人子宮內(nèi)膜癌細(xì)胞 5 × 105 1-2weeks
SKOV3 人卵巢癌細(xì)胞 5 × 105 1-2weeks
HO-8910 人卵巢癌細(xì)胞 5 × 105 1-2weeks
SK-OV-3 人卵巢腺癌細(xì)胞 5 × 105 1-2weeks
OVCAR-3 人卵巢腺癌細(xì)胞 5 × 105 1-2weeks
JEG3 人絨毛膜癌細(xì)胞 5 × 105 1-2weeks
Ms751 人子宮頸表皮癌細(xì)胞 5 × 105 1-2weeks
Siha 人子宮頸鱗癌細(xì)胞 5 × 105 1-2weeks
KLE 人子宮內(nèi)膜腺癌細(xì)胞 5 × 105 1-2weeks
消化系統(tǒng)digestive system
PANC-1細(xì)胞，人胰腺癌細(xì)胞 5 × 105 1-2weeks
SW1990細(xì)胞， 人胰腺癌細(xì)胞 5 × 105 1-2weeks
BxPC-3細(xì)胞，人胰腺腺癌細(xì)胞 5 × 105 1-2weeks
chang liver細(xì)胞， 人張氏肝細(xì)胞 5 × 105 1-2weeks
HIC-2細(xì)胞， 人小腸癌細(xì)胞 5 × 105 1-2weeks
HCT-8細(xì)胞，人回盲腸癌細(xì)胞 5 × 105 1-2weeks
SW620[SW-620] 細(xì)胞，人結(jié)腸癌細(xì)胞 5 × 105 1-2weeks
HCT-116細(xì)胞，人結(jié)腸癌細(xì)胞 5 × 105 1-2weeks
COLO 205 細(xì)胞，人結(jié)腸癌細(xì)胞 5 × 105 1-2weeks
RKO細(xì)胞，人結(jié)腸腺癌細(xì)胞 5 × 105 1-2weeks
LoVo細(xì)胞，人結(jié)直腸腺癌細(xì)胞 5 × 105 1-2weeks
HT-29細(xì)胞， 人結(jié)直腸腺癌細(xì)胞 5 × 105 1-2weeks
Caco-2細(xì)胞，人結(jié)直腸腺癌細(xì)胞 5 × 105 1-2weeks
SW480細(xì)胞，人結(jié)直腸腺癌細(xì)胞 5 × 105 1-2weeks
SW620細(xì)胞，人結(jié)直腸腺癌細(xì)胞 5 × 105 1-2weeks
SW837細(xì)胞，人結(jié)直腸腺癌上皮細(xì)胞 5 × 105 1-2weeks
DLD-1細(xì)胞， 人結(jié)直腸腺癌上皮細(xì)胞 5 × 105 1-2weeks
KYSE30細(xì)胞，人食管鱗癌細(xì)胞 5 × 105 1-2weeks
KYSE-150 細(xì)胞，人食管鱗癌細(xì)胞 5 × 105 1-2weeks
KYSE-450細(xì)胞，人食管鱗癌細(xì)胞 5 × 105 1-2weeks
TE-1 細(xì)胞，人食管癌細(xì)胞 5 × 105 1-2weeks
ECA109細(xì)胞，人食管癌細(xì)胞 5 × 105 1-2weeks
EC9706細(xì)胞，人食管癌細(xì)胞 5 × 105 1-2weeks
MH97H細(xì)胞，人高轉(zhuǎn)移潛能肝癌細(xì)胞株 5 × 105 1-2weeks
HCCLM3細(xì)胞， 人高轉(zhuǎn)移肝癌細(xì)胞 5 × 105 1-2weeks
SMMC-7721細(xì)胞，人肝癌細(xì)胞 5 × 105 1-2weeks
BEL-7404 細(xì)胞，人肝癌細(xì)胞 5 × 105 1-2weeks
Hep G2細(xì)胞，人肝癌細(xì)胞 5 × 105 1-2weeks
Hep-3B細(xì)胞，人肝癌細(xì)胞 5 × 105 1-2weeks
BEL-7402 細(xì)胞，人肝癌細(xì)胞 5 × 105 1-2weeks
BEL-7404 細(xì)胞，人肝癌細(xì)胞 5 × 105 1-2weeks
SNU-739 細(xì)胞，人肝癌細(xì)胞 5 × 105 1-2weeks
HepG2細(xì)胞，人肝癌細(xì)胞 5 × 105 1-2weeks
97L細(xì)胞，人肝癌細(xì)胞 5 × 105 1-2weeks
HuH-7細(xì)胞， 人肝癌細(xì)胞 5 × 105 1-2weeks
QGY-7703細(xì)胞，人肝癌細(xì)胞 5 × 105 1-2weeks
HOC細(xì)胞， 肝卵圓細(xì)胞 5 × 105 1-2weeks
L-02（HL-7702) 細(xì)胞， 人正常肝細(xì)胞 5 × 105 1-2weeks
QSG-7701細(xì)胞，人正常肝細(xì)胞 5 × 105 1-2weeks
GES-1細(xì)胞， 人胃上皮細(xì)胞 5 × 105 1-2weeks
MGC-803細(xì)胞，人胃癌細(xì)胞 5 × 105 1-2weeks
MKN-45細(xì)胞，人胃癌細(xì)胞 5 × 105 1-2weeks
AGS細(xì)胞， 人胃癌細(xì)胞 5 × 105 1-2weeks
AZ-521細(xì)胞，人胃癌細(xì)胞 5 × 105 1-2weeks
Hs 746T細(xì)胞，人胃癌細(xì)胞 5 × 105 1-2weeks
NUGC-3細(xì)胞，人胃癌細(xì)胞 5 × 105 1-2weeks
SNU-638細(xì)胞，人胃癌細(xì)胞 5 × 105 1-2weeks
NCI-N87細(xì)胞，人胃癌細(xì)胞 5 × 105 1-2weeks
HGC-27細(xì)胞，人胃癌細(xì)胞（未分化） 5 × 105 1-2weeks
BGC-823 細(xì)胞，人胃腺癌細(xì)胞 5 × 105 1-2weeks
SGC-7901 細(xì)胞，人胃腺癌細(xì)胞 5 × 105 1-2weeks
GBC-SD細(xì)胞，人膽囊癌細(xì)胞 5 × 105 1-2weeks
QBC939細(xì)胞，人膽管癌細(xì)胞 5 × 105 1-2weeks
血液和淋巴系統(tǒng)Blood and lymphatic system
6TCEM細(xì)胞，人T細(xì)胞白血病細(xì)胞 5 × 105 1-2weeks
JURKAT細(xì)胞，人T淋巴細(xì)胞白血病細(xì)胞株 5 × 105 1-2weeks
CCRF-CEM細(xì)胞， 人急性淋巴細(xì)胞白血病T淋巴細(xì)胞 5 × 105 1-2weeks
MOLT-4細(xì)胞，人急性淋巴母細(xì)胞白血病細(xì)胞 5 × 105 1-2weeks
REH 細(xì)胞，急性淋巴細(xì)胞白血病 5 × 105 1-2weeks
supb15細(xì)胞，急淋白血病細(xì)胞系 5 × 105 1-2weeks
K-562細(xì)胞， 人慢性髓原白血病細(xì)胞 5 × 105 1-2weeks
K562細(xì)胞，人慢性髓性白血病細(xì)胞 5 × 105 1-2weeks
HL-60細(xì)胞， 白血病細(xì)胞 5 × 105 1-2weeks
TF-1細(xì)胞，人血液白血病細(xì)胞 5 × 105 1-2weeks
HSPC-1細(xì)胞，人造血干細(xì)胞 5 × 105 1-2weeks
Daudi細(xì)胞， 人Burkitt淋巴瘤細(xì)胞 5 × 105 1-2weeks
NAMALWA細(xì)胞， 人Burkitt淋巴瘤細(xì)胞 5 × 105 1-2weeks
Raji細(xì)胞 人Burkitt淋巴瘤細(xì)胞 5 × 105 1-2weeks
RAMOS細(xì)胞，人B淋巴細(xì)胞瘤細(xì)胞 5 × 105 1-2weeks
HUVEC[HUV-EC-C] 細(xì)胞， 人臍靜脈內(nèi)皮細(xì)胞 5 × 105 1-2weeks
MPCs細(xì)胞， 單核巨噬細(xì)胞 5 × 105 1-2weeks
THP-1細(xì)胞， 急性單核白血病細(xì)胞 5 × 105 1-2weeks
U937細(xì)胞， 人組織細(xì)胞淋巴瘤細(xì)胞 5 × 105 1-2weeks
HCAEC細(xì)胞，人冠狀動(dòng)脈內(nèi)皮細(xì)胞 5 × 105 1-2weeks
HemSc細(xì)胞，血管瘤干細(xì)胞 5 × 105 1-2weeks
人淋巴細(xì)胞 5 × 105 1-2weeks
循環(huán)系統(tǒng)circulatory system
HUV-EC細(xì)胞，人臍靜脈內(nèi)皮細(xì)胞 5 × 105 1-2weeks
眼、耳、鼻、喉和口腔系統(tǒng)Eye, ear, nose, throat and mouth system
CAL-27細(xì)胞，人舌鱗癌細(xì)胞 5 × 105 1-2weeks
Tca-8113細(xì)胞， 人舌鱗癌細(xì)胞 5 × 105 1-2weeks
HDPC細(xì)胞， 人牙髓細(xì)胞 5 × 105 1-2weeks
人牙周膜細(xì)胞 5 × 105 1-2weeks
PDLC細(xì)胞， 牙周膜成纖維細(xì)胞 5 × 105 1-2weeks
CNE-2Z細(xì)胞，人鼻咽癌細(xì)胞 5 × 105 1-2weeks
人耳周膜細(xì)胞 5 × 105 1-2weeks
SCC-9細(xì)胞， 人口腔癌細(xì)胞 5 × 105 1-2weeks
KB細(xì)胞，人口腔表皮樣癌細(xì)胞 5 × 105 1-2weeks
收到處理方式：
新鮮細(xì)胞到貨處理方式，客戶收到細(xì)胞后按以下方式操作：
取出25cm2培養(yǎng)瓶，75%酒精消毒，拆下封口膜，放入37℃，5% CO2細(xì)胞培養(yǎng)箱中靜置2-3h，以穩(wěn)定細(xì)胞狀態(tài)。
待細(xì)胞達(dá)到80%匯合時(shí)準(zhǔn)備進(jìn)行傳代培養(yǎng)。
細(xì)胞傳代，吸出25cm2培養(yǎng)瓶中的培養(yǎng)基,用PBS清洗細(xì)胞一次；添加0.25%胰蛋白酶消化液約1ml至培養(yǎng)瓶中，37℃溫浴3min左右；倒置顯微鏡下觀察，待細(xì)胞回縮變圓后吸棄消化液，再加入*培養(yǎng)液終止消化；用吸管輕輕吹打混勻，按1:2適當(dāng)?shù)谋壤M(jìn)行接種傳代（原代細(xì)胞必須以1:2方式傳代），然后補(bǔ)充新鮮的*培養(yǎng)基至5ml，放入37℃，5% CO2細(xì)胞培養(yǎng)箱中培養(yǎng)；待細(xì)胞*貼壁后，培養(yǎng)觀察。之后每隔2-3天更換新鮮的*培養(yǎng)基。
凍存管到貨后處理方式，客戶收到細(xì)胞產(chǎn)品后，請(qǐng)按照下述方法操作：
細(xì)胞凍存管公司多贈(zèng)送一管，建議客戶先復(fù)蘇1支，如果復(fù)蘇效果不理想，先與技術(shù)人員溝 通后再復(fù)蘇另1支。
細(xì)胞收到后盡快復(fù)蘇，有效期為一周。
收到凍存管后如不馬上復(fù)蘇，可以暫時(shí)放置于-80℃冰箱，不建議放入液氮罐。
復(fù)蘇所用血清必須是胎牛血清。
復(fù)蘇請(qǐng)按照細(xì)胞復(fù)蘇 SOP 方式操作（細(xì)胞復(fù)蘇 SOP 另行發(fā)送）。
熱賣：DC2.4細(xì)胞，SW48細(xì)胞，RS4:11細(xì)胞，KGN細(xì)胞，A2780細(xì)胞，A2780/DDP細(xì)胞，HL-1細(xì)胞，COV434細(xì)胞等
更多人卵巢癌細(xì)胞系、人肺癌細(xì)胞系、人結(jié)腸癌細(xì)胞系、人肝癌細(xì)胞系、人膀胱癌細(xì)胞系 耐藥株 盡在慧穎細(xì)胞庫
訂購：
：
2452981082
：王
公司地址：上海市嘉定區(qū)眾仁路399號(hào)運(yùn)通星財(cái)富廣場(chǎng)A座202室
