您好, 歡迎來到化工儀器網(wǎng)! 登錄| 免費(fèi)注冊(cè)| 產(chǎn)品展廳| 收藏商鋪|
當(dāng)前位置:> 供求商機(jī)> GP2d細(xì)胞,人結(jié)腸癌細(xì)胞系
貨物所在地:上海上海市
產(chǎn)地:上海/國外引進(jìn)
更新時(shí)間:2024-05-27 21:00:07
有效期:2024年5月27日 -- 2024年11月27日
已獲點(diǎn)擊:173
聯(lián)系我時(shí),請(qǐng)告知來自 化工儀器網(wǎng)
產(chǎn)品名稱:GP2d細(xì)胞,人結(jié)腸癌細(xì)胞系
同類人結(jié)腸癌細(xì)胞系有:SW480細(xì)胞、SW48細(xì)胞、Caco2細(xì)胞、SW620細(xì)胞、HCT116細(xì)胞、 HCT8細(xì)胞、HT29細(xì)胞、LS174T細(xì)胞、LOVO細(xì)胞、GP2d細(xì)胞
關(guān)于 GP2d細(xì)胞,人結(jié)腸癌細(xì)胞系 詳細(xì)介紹有:
Medium & See Propagation
Organism: Homo sapiens (human)
Source: Organ: colon
Disease: colorectal adenocarcinoma
Morphology: epithelial
Growth adherent
Properties:
Cellular carcinoembryonic antigen (CEA)
Products:
Products
Age: 71 years
Gender: female
Propagation: The base medium for this cell line is formulated RPMI-1640 Medium. To make the complete
growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C
Subculturing: Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 1 to 5 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37癈 to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C
Preservation: culture medium 95%; DMSO, 5%
來源:慧穎生物實(shí)驗(yàn)室
訂購:/8
:王
以下為我實(shí)驗(yàn)室主要供應(yīng)的癌細(xì)胞系,每天細(xì)胞系數(shù)目還在增加,如您在此未查到您所需要的細(xì)胞株細(xì)胞系名稱,請(qǐng)致電我公司咨詢,感謝關(guān)注。
請(qǐng)輸入賬號(hào)
請(qǐng)輸入密碼
請(qǐng)輸驗(yàn)證碼
以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),化工儀器網(wǎng)對(duì)此不承擔(dān)任何保證責(zé)任。
溫馨提示:為規(guī)避購買風(fēng)險(xiǎn),建議您在購買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。