貨號(hào)K1622 RevertAid First Strand cDNA Synthesis Kit 100 reactions
K1622逆轉(zhuǎn)錄酶試劑盒 Thermo反轉(zhuǎn)錄試劑盒
RevertAid First Strand cDNA Synthesis Kit
貨號(hào): K1622
貨號(hào) 單位規(guī)格 目錄價(jià)格 (CNY)
K1622 100 reactions 1,951.00
K1622逆轉(zhuǎn)錄酶試劑盒 Thermo反轉(zhuǎn)錄試劑盒
K1622產(chǎn)品描述
Thermo Scientific RevertAid*鏈cDNA合成試劑盒包含了足夠進(jìn)行100次x20 μL反應(yīng)的試劑。本品是以RNA為模板高效合成*鏈cDNA的完整系統(tǒng),可合成長(zhǎng)達(dá)13kb的cDNA。本品含有RiboLock RNase抑制劑,能夠有效保護(hù)RNA模板不受降解。
Thermo Scientific RevertAid*鏈cDNA合成試劑盒配套提供oligo(dT)18引物和隨機(jī)六聚體引物。oligo(dT)18引物可以選擇性地與mRNA中的poly (A)尾結(jié)合。隨機(jī)六聚體引物無(wú)需poly (A)尾,因此可轉(zhuǎn)錄mRNA 5’-端區(qū)域,也可以無(wú)poly (A)尾的RNA (如micoRNA)為模板合成cDNA。該試劑盒也可使用基因特異性引物。
特點(diǎn):
• 可以高效合成長(zhǎng)達(dá)13kb的全長(zhǎng)*鏈cDNA
• *活性溫度為42°C
• *一提供RT反應(yīng)所需的所有組分
應(yīng)用
• *鏈cDNA合成,作為RT-PCR和實(shí)時(shí)RT-qPCR的模板
• 構(gòu)建全長(zhǎng)cDNA文庫(kù)
• 反義RNA合成
For Research Use Only. Not for use in diagnostic procedures.
K1622逆轉(zhuǎn)錄酶試劑盒 Thermo反轉(zhuǎn)錄試劑盒
K1622規(guī)格
Reverse Transcriptase: | RevertAid Reverse Transcriptase |
---|---|
Downstream Application: | Reverse Transcriptase PCR (RT-PCR), Real-Time Quantitative PCR (qPCR) |
Reaction Format: | Separate Components |
Sample Type (General): | RNA |
Product Size: | 100 reactions |
Optimal Reaction Temperature: | 42° C |
k1622內(nèi)容及儲(chǔ)存
The RevertAid First Strand cDNA Synthesis Kit contains RevertAid Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, Oligo(dT)18 Primer, Random Hexamer Primer, Control GAPDH RNA, 10 μM Forward GAPDH Primer, 10 μM Reverse GAPDH Primer, and nuclease-free water.
Store at -20°C.