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檢測(cè)限和靈敏度zui高的植物乙烯測(cè)量系統(tǒng)

超高靈敏度測(cè)量，特別是連續(xù)監(jiān)測(cè)

主要功能

該系統(tǒng)主要用于植物研究的乙烯監(jiān)測(cè)，如生長(zhǎng)發(fā)育、基因表達(dá)、植物病原體的相互作用、與其他植物激素的相互作用、蔬果收貨后儲(chǔ)存、抗逆性研究（干旱、高溫、重金屬）等。其中檢測(cè)儀ETD-300結(jié)合激光技術(shù)與聲學(xué)照相技術(shù)，實(shí)時(shí)快速測(cè)量乙烯（C₂H₄）氣體濃度；閥門(mén)控制箱VC-6*自動(dòng)化和電腦控制，接一個(gè)即可以使單個(gè)氣體檢測(cè)儀實(shí)現(xiàn)6個(gè)樣品的同時(shí)測(cè)量，單個(gè)乙烯檢測(cè)儀可以接一個(gè)或多個(gè)閥門(mén)控制箱；烴分解器CAT-1則利用鉑金顆粒催化烴氧化分解為水蒸氣和CO₂，為系統(tǒng)提供無(wú)烴干擾的樣品空氣。

應(yīng)用領(lǐng)域

用于環(huán)境、醫(yī)學(xué)、農(nóng)業(yè)、工業(yè)、生態(tài)、生物等監(jiān)測(cè)領(lǐng)域。特別適合植物生理、發(fā)育研究的超靈敏乙烯測(cè)量。

產(chǎn)品特點(diǎn)

● 實(shí)時(shí)測(cè)量，連續(xù)無(wú)間斷監(jiān)測(cè)

● 測(cè)量范圍：0-5ppmv，檢出限：0.3ppbv

● 靈敏、快速、穩(wěn)定性好，無(wú)干擾

● 操作簡(jiǎn)便，結(jié)實(shí)耐用，所需維護(hù)少

● 友好的用戶操作軟件界面

系統(tǒng)組成

乙烯氣體檢測(cè)儀ETD-300

閥門(mén)控制箱VC-6

烴分解器CAT-1

注：系統(tǒng)中3個(gè)儀器都可以單獨(dú)使用。

主要技術(shù)參數(shù)

參數(shù)	乙烯氣體檢測(cè)儀ETD-300	閥門(mén)控制箱VC-6	烴分解器CAT-1
測(cè)量范圍	0-5 ppmv	/	/
檢出限	0.3 ppbv	/	/
噪音（2σ）	0.3 ppbv	/	/
精度	<1% 或 0.3 ppbv	0.2% FS	/
穩(wěn)定性	<1%超過(guò)24小時(shí)	/	/
零點(diǎn)漂移	+/-1 ppbv	/	/
測(cè)量時(shí)間	5 s	/	/
響應(yīng)時(shí)間	30 s （當(dāng)流量為1 l/h時(shí)）	300 ms	/
流量	0.25-5 l/h	0.25-5 l/h	0-30 l/h
校準(zhǔn)	使用標(biāo)準(zhǔn)混合氣，每年一次	/	/
通道數(shù)量	/	6（可增加至12, 18等）	/
測(cè)定模式	/	連續(xù)流動(dòng)測(cè)定，積累后測(cè)定	/
氣體供應(yīng)壓力	/	0.5-4 Bar	/
過(guò)壓閥		在5 Bar時(shí)打開(kāi)	/
濾膜類型	/	粒徑>7µm	/
zui大稀釋濃度	/	/	100 ppm
輸出濃度	/	/	< 100 pptv
壓力	/	/	0-6 atm
活性催化劑	/	/	Pt/SiO₂
催化溫度	/	/	150 – 250 ℃
預(yù)熱時(shí)間	30 min	/	< 10 min
尺寸	50x50x14cm （48.3cm 3U機(jī)架）	40x50x10cm （48.3cm 2U機(jī)架）	40x25x20cm （48.3cm 3U半機(jī)架）
工作溫度/濕度	10-28 ℃/0-95 % RH	5-40℃/0-95 % RH	5-40℃ /0-95 %RH
電源要求	90-264 VAC, 47-63 Hz	90-264 VAC, 47-63 Hz	90-264 VAC, 47-63 Hz
功耗	<150 W	<60 W	85 W
進(jìn)氣接口	1/8'' Swagelok	用于接外徑1/8''的管	1/8'' Swagelok
數(shù)據(jù)輸出	USB 和 RS232，CSV格式	USB，CSV格式	/
顯示	藍(lán)色背光LCD	LED指示燈	/

部分利用ETD發(fā)表的文獻(xiàn)

1. Cristescu S.M., Persijn S.T., te Lin Hekkert S., Harren F.J.M. Laser-based systems for trace gasdetection in life sciences, （2008）, Appl. Phys. B 92, 343-349

2. de Gouw J.A., Hekkert S. T. L., Mellqvist J., Warneke C., Atlas E.L., Fehsenfeld F.C., Fried A., Frost G.J., Harren F.J.M., Holloway J.S., Lefer B., Lueb R., Meagher J.F., Parrish D.D., Pa M., Pope L., Richter D., Rivera C., Ryerson T.B., Samuelsson J., Walega J., Washenfelder R.A., Weibring P., Zhu X., Airborne Measurements of Ethene from Industrial Sources Using Laser Photo-Acoustic Spectroscopy, （2009）, Environ. Sci. Technol. 43, 2437–2442

3. McDonnell L., Plett J.M., Andersson-Gunneras S., Kozela J.D., Dominique V.D. S., Bernard R.G., Björn S., Sharon R., Ethylene levels are regulated by a plant encoded 1-aminocyclopropane-1-carboxylic acid deaminase, （2009）, Physilogia Plantarum 136, 94-109

4. Clarke S.M., Cristescu S.M., Miersch O., Harren F.J. M., Wasternack C., Mur L.A. J., Jasmonates act with salicylic acid to confer basal thermotolerance in Arabidopsis thaliana, （2009）, New Phytologist 182:175-187

5. Hermans C., Vuylsteke M., Cristescu S.M., InzéD., Verbruggen N., Systems analysis of the responses to long term magnesium, deficiency and restoration in Arabidopsis thaliana highlights a possible role of the circadian clock in the plant adaptation, （2010）, New Phytologist 187: 132-144

6. Cristescu S.M., de Martinis D., te Lin Hekkert S., Parker D.H., Harren F.J.M., Ethylene production by Botrytis cinerea in vitro and in tomato fruit , （2002）, Applied and Environmental Microbiology 68, 5342-5350

7. Cristescu S.M., Woltering E.J., Harren F.J.M., Real time monitoring of ethylene during fungal-plant interaction by laser-based photoacoustic spectroscopy in “Food Mycology, A Multifaceted Approach to Fungiand Food”, （2006）, J. Dijksterhuis and R.A. Samson, Eds., Traylor and Francis New York, USA 25-47

8. Staal M., Stal L.J., te Lin-Hekkert S., Harren F.J.M., Light action spectra of N₂ fixation by heterocystous cyanobacteria from the Baltic Se, （2003）, Journal of Phycology 39, 668-677

9. Severin, J Stal L., Light dependency of nitrogen fixation in a coastal cyanobacterial mat, （2008）, The ISME Journal 2, 1077–1088; doi:10.1038/ismej.2008.63

10. Photoacoustic trace gas detection of ethene released by UV-induced lipid peroxidation in humans, S. Cristescu, R. Berkelmans, S. te Lin Hekkert, B. Timmerman, D. Parker, F. Harren, Proceedings SPIE Vol. 4162 （Bellingham, USA, 2000） 101-107

11. Moeskops B.W.M., Steeghs M.M.L., van Swam K., Cristescu S.M., Scheepers P.T.J., Harren F.J.M., “Realtime trace gas sensing of ethylene, propanal and acetaldehyde from human skin in vivo”. （2006）, Physiological Measurement 27, 1187-1196

12. Roeder S., Dreschler K., Wirtz M., Cristescu S.M., Harren F.J.M., Hell R., Piechulla B.. SAM levels, gene expression of SAM synthetase, methionine synthase and ACC oxidase, and ethylene emission from N. suaveolens flowers. （2009）, Molecular Biology 70, 535-546

13. Salman A., Filgueiras H., Cristescu S., Lopez-Lauri F., Harren F., Sallanon H., Inhibition of wound-induced ethylene does not prevent red discoloration in fresh-cut endive （Cichorium intybus L.）. （2009）, Eur. Food Res. Technol. 228, 651-657

14. Mur L. A.J., Lloyd A.J., Cristesc S.M.u, Harren F.J.M., Hal M.l., Smith A., Biphasic ethylene production during the hypersensitive response in Arabidopsis: A window into defence priming mechanisms? （2009）, Plant Signalling & Behaviour 4 （7）, 610 – 613

15. Yordanova Z.P., Iakimova E.T., Cristescu S.M., Harren F.J. M., Kapchina-Toteva V.M., Woltering E.J., Involvement of ethylene and nitric oxide in cell death in mastoparan-treated unicellular alga Chlamydomonas reinhardtii. （2010）, Cell Biology International 34, 301-308

16. Benlloch-González M., Romera J., Cristescu S., Harren F., María Fournier J., Benlloch M., K+ starvation inhibits water-stress-induced stomatal closure via ethylene synthesis in sunflower plants, （2010）, Journal of Experimental Botany 61, 1139 – 1145

17. Yordanova Z.P., Kapchina – Toteva V.M., Woltering E.J., Cristescu S.M., Harren F.J.M., Iakimova E.T., Mastoparan- induced cell death signaling in Chlamydomonas Reinhardtii. （2009）, Biotechnology & Biotechnological equipment-special issue, Vol.23: 730-734

18. Hélène Lequeux, Christian Hermans, Stanley Lutts, Nathalie Verbruggen, Response to copper excess in Arabidopsis thaliana: Impact on the root system architecture, hormone distribution, lignin accumulation and mineral profile, （2010）, Plant Physiology and Biochemistry 48, 673-682

19. Lloyd A.J., William Allwood J., Winder C., Dunn W.B., Heald J., Cristescu S.M., Harren F.J. M., Goo-dacre R., Smith A.R., Mur L.A. J., Metabolomic approaches indicate that cell wall modifications play a major role in ethylene-mediated resistance against Botrytis cinerea, （2011）,Plant Journal 67（5）, 852-868.

20. Moniuszko G., Laska-Oberndorff A., Cristescu S.M., Harren F.J.M., Sirko A., Ethylene emitted by nylon membrane filters questions their usefulness to transfer plant seedlings between media, （2011）, BioTechniques 51:doi 10.2144/000113762

21. Gallego-Bartolomé J., Arana M.V., Vandenbussche F., ?ádníková P., Minguet E.G., Guardiola V., Van Der Straeten D., Benkova E., AlabadíD., Blázquez M. A., Hierarchy of hormone action controlling apical hook development in Arabidopsis, （2011）, The Plant Journal 67, 622–634

22. Liesbeth D.G., Laury C., Jasper D., Jan D., Ivo R., Wim H. V., Thomas M., Gerrit T. S. B., Andy L. P., Nicholas P. H., Peter H., Dominique V.D.S., Blackwell Publishing Ltd Reduced gibberellin response affects ethylene biosynthesis and responsiveness in the Arabidopsis gai eto2-1 double mutant, （2008）, New Phytologist 177, 128–141

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