采用常規(guī)生化方法，通過標準曲線和比色，可定量分析總硫酸化多糖（氨基聚糖和蛋白聚糖）的含量，亦可測定O端和N端硫酸氨基多糖的比值。主要采用的是Blyscan染料（Biocolor公司的產(chǎn)品），它可以特異性的與氨基聚糖和蛋白聚糖上的硫酸多糖組分結合。

Biocolor 硫酸氨基聚糖檢測試劑盒 
Biocolor Blyscan™ Glycosaminoglycan Assay
 
    采用常規(guī)生化方法，通過標準曲線和比色，可定量分析總硫酸化多糖（氨基聚糖和蛋白聚糖）的含量，亦可測定O端和N端硫酸氨基多糖的比值。主要采用的是Blyscan染料（Biocolor公司的產(chǎn)品），它可以特異性的與氨基聚糖和蛋白聚糖上的硫酸多糖組分結合。該方法不適于檢測已經(jīng)降解的硫酸化雙糖片段。
    該試劑盒可檢測廣泛的蛋白多糖，可檢測的氨基多糖包括硫酸軟骨素、硫酸角質素、硫酸皮膚素、硫酸肝素，不適合檢測包含海藻酸的樣品，因為它們含有糖醛酸。該試劑盒也不能對透明質酸定量，但透明質酸并不干擾對硫酸化氨基多糖的檢測。細胞培養(yǎng)基中20%的胎牛血清也不會干擾實驗。該方法檢測靈敏度為5ug，所需時間為1小時。
 
檢測樣本：可來自軟骨組織、動脈組織、肺組織、皮膚組織、腫瘤組織、細胞核組織提取物、細胞培養(yǎng)液、羊水和尿液
 
實驗操作 
1. 繪制標準曲線
2. 待測樣品的處理：
取50ul待測樣品進行實驗。如果反應物在656nm初的吸收峰大于1.5，則對樣品進行稀釋，待測樣樣品濃度落入標準曲線中段時，準確度較高。
3. 硫酸氨基聚糖—染料復合物的獲得
加1.0ml的Blyscan染料溶液到待測樣本中，充分混勻，待不溶物出現(xiàn)，離心，棄上清，則獲得紫色的硫酸氨基聚糖—染料復合物
4. 硫酸氨基聚糖—染料復合物中染料的釋放
加入1.0ml的染料釋放劑，充分混勻。溶解5min以上，使染料充分釋放
5. 測定
用分光光度計于波長656nm處測定，或使用colorimeter紅色濾光片測定
 
產(chǎn)品訂購

   Product Code: B1000     110 assays 
   Product Code: B3000     440 assays

 

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