表面活性物質(zhì)關(guān)聯(lián)蛋白B(SPB)檢測(cè)試劑盒

適用生物 Homo sapiens (Human，人)
表面活性物質(zhì)關(guān)聯(lián)蛋白B(SPB)檢測(cè)試劑盒檢測(cè)范圍 1.56-100ng/mL 靈敏度 0.61ng/mL
樣本類型 Serum, plasma, lung lavage fluid, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
實(shí)驗(yàn)時(shí)長(zhǎng) 4.5h 實(shí)驗(yàn)方法 雙抗夾心法 表面活性物質(zhì)關(guān)聯(lián)蛋白B(SPB)檢測(cè)試劑盒規(guī)格 96T

ELISA Kit for Surfactant Associated Protein B (SPB)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

表面活性物質(zhì)關(guān)聯(lián)蛋白B(SPB)檢測(cè)試劑盒Specificity

This assay has high sensitivity and excellent specificity for detection of Surfactant Associated Protein B (SPB).
No significant cross-reactivity or interference between Surfactant Associated Protein B (SPB) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Surfactant Associated Protein B (SPB) and the recovery rates were calculated by comparing the measured value to the expected amount of Surfactant Associated Protein B (SPB) in samples.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Surfactant Associated Protein B (SPB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Surfactant Associated Protein B (SPB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

表面活性物質(zhì)關(guān)聯(lián)蛋白B(SPB)檢測(cè)試劑盒Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Surfactant Associated Protein B (SPB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100μL standard or sample to each well. Incubate 2 hours at 37^oC;
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37^oC;
4. Aspirate and wash 3 times;
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37^oC;
6. Aspirate and wash 5 times;
7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37^oC;
8. Add 50μL Stop Solution. Read at 450nm immediay.

表面活性物質(zhì)關(guān)聯(lián)蛋白B(SPB)檢測(cè)試劑盒Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Surfactant Associated Protein B (SPB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Surfactant Associated Protein B (SPB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Surfactant Associated Protein B (SPB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Surfactant Associated Protein B (SPB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.