鼠抗人CD71單克隆抗體

廣州健侖生物科技有限公司

轉(zhuǎn)鐵蛋白受體CD71在胎盤合體滋養(yǎng)細胞、肌細胞、肝細胞、精母細胞、幼稚紅細胞等有高水平表達，其中在幼稚紅細胞早期至正常紅細胞的過渡期表達zui高，隨著紅細胞的成熟，轉(zhuǎn)鐵蛋白受體CD71表達量隨之下降。由于幼稚紅細胞中CD71的特性表達使CD71抗體成為一個有價值的作為骨髓中幼稚紅細胞成分的參考依據(jù)。CD71抗體在骨髓組織中特異性地標(biāo)記幼稚紅細胞，而成熟紅細胞，和其它造血細胞幾乎不表達CD71，所以，免疫組織化學(xué)檢測CD71抗體是紅血球性白血病、良性紅細胞增生性紊亂和骨髓發(fā)育不良癥的重要參考依據(jù)。

我司還提供其它進口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【產(chǎn)品介紹】

細胞定位：細胞漿/細胞膜

克隆號：MRQ-48

同型：IgG1

適用組織：石蠟/冰凍

陽性對照：骨髓

抗原修復(fù)：熱修復(fù)（EDTA）

抗體孵育時間：30-60min

討論
1.小鼠生殖細胞單細胞懸液制備的影響因素
良好單細胞懸液的制備必須首先考慮所用小鼠的日齡。因為隨動物日齡的增加，精原細胞開始分化形成各級生殖細胞，其數(shù)目及所占生殖細胞的比例都逐漸下降，會給分離純化增加困難。在成年小鼠的睪丸中，A型精原細胞只占所有精原細胞的1.25%，占未分化精原細胞的10.6%，僅占所有生殖細胞的0.03%［5］。因而用來分離精原細胞的動物的日齡應(yīng)較小為好。一般小鼠選用生后8d的［2］，但也有人選用生后10d的；大鼠則選用生后9d的［6］。根據(jù)我們的前期工作并參考有關(guān)報道［2，3］，7～8d小鼠的生精上皮內(nèi)基本只有A型精原細胞和支持細胞，其他各級生精細胞尚未發(fā)育形成，理論上可獲得zui大量的精原細胞。
其次，采用適當(dāng)?shù)某绦蚣跋椒ㄒ彩侵苽淞己脝渭毎麘乙旱年P(guān)鍵環(huán)節(jié)之一。從小鼠體內(nèi)取出睪丸之后，我們先用尖鑷仔細去除脂肪墊及睪丸白膜，將曲細精管在PBS中吹散，盡量將間質(zhì)吹打成單細胞及組織碎片。通過靜置或低速離心，將其去除，獲得較為純粹的曲細精管段。然后采用兩次較短時間的膠原酶消化，將沒有消散的睪丸間質(zhì)消散并釋放大部分管周肌樣細胞，zui大程度地減少了間質(zhì)組織及管周肌樣細胞的污染。zui后采用胰蛋白酶、EDTA復(fù)合消化液消化，釋放支持細胞和精原細胞。實驗結(jié)果表明，這種程序性的消化過程能有效地分離到較為純粹的曲細精管段并制備出較高產(chǎn)量和活率的單細胞懸液，同時確保絕大部分間質(zhì)成分，管周肌樣細胞及睪丸外細胞的去除。
2.Percoll不連續(xù)密度梯度的分離效果

我司還提供其它進口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】     歐

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

discuss
1 mouse germ cell single cell suspension preparation of the influencing factors
The preparation of a good single cell suspension must first consider the age of the mice used. Because with the increase of animal age, spermatogonial cells begin to differentiate to form germ cells at all levels, the absolute number and proportion of germ cells are gradually decreased, will increase the difficulty of separation and purification. In testis of adult mice, type A spermatogonia account for only 1.25% of all spermatogonia, accounting for 10.6% of undifferentiated spermatogonia, accounting for only 0.03% of all germ cells [5]. Therefore, the animal used to isolate spermatogonia should be as small as possible. General mice selected 8d after birth [2], but also some people choose after birth 10d; rats were selected 9d after birth [6]. According to our previous work and with reference to relevant reports [2, 3], there are only type A spermatogonia and supporting cells in the seminiferous epithelium of 7 ~ 8 days mice, and other spermatogenic cells at all levels have not yet developed and are theoretically available The largest amount of spermatogonia.
Second, using proper procedures and digestion methods is also one of the key steps in preparing a good single cell suspension. After removing the testis from the body of the mouse, we carefully remove the fat pad and the testicular white membrane with sharp tweezers, blow the seminiferous tubules in PBS, and blow the interstitial into single cells and tissue fragments as much as possible. By standing or low-speed centrifugation, it is removed to obtain a more pure seminiferous tubule. Then using two shorter collagenase digestion, dissipate the testicular interstitium and release most of the peritubular myeloid cells, to minimize the interstitial and peritubular myoid-like cell contamination. Finally, trypsin, EDTA digestion of digestion, the release of supporting cells and spermatogonia. The experimental results show that this process of digestion can effectively separate the more pure seminiferous tubules and produce a single cell suspension with higher yield and viability while ensuring that most interstitial components, Like cells and testicular cells removed.
2.Percoll discrete density gradient separation effect