F98 大鼠膠質(zhì)瘤細(xì)胞

Catalogue No.: C1035

Product Format: a T25 flask

Culture Properties：貼壁

Complete Growth Medium: 89%H-DMEM+10%FBS+1%雙抗

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Application: Cells and cancer research

NOTE: FOR RESEARCH USE ONLY.

Components

For LC3 staining, 1-h ?xation and permeabilization against exposure to light at room temperature was required after FVS780 staining. Then, the cells were washed twice with perm/wash buffer, and Alexa Fluor 488-labelled LC3 antibody (Cell Signalling Technology, USA) in perm/ wash buffer was mixed with the ?xed cells at a dilution of 1:50 for 1 h at room temperature. After discarding of the